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Further, we observed beating by the two separated hearts in the fn;spns2 mutant (supplementary material Movies 1, 2).
We found the two separated hearts phenotype in the fn;spns2 double mutant was more severe than that in either fn mutant or spns2 mutants.
As shown in Fig. 1, the fn:spns2 double mutant displayed two widely separated hearts (19/322 embryos obtained from the crossing of fn kt259 spns2 ko157 heterozygous fish).
In zebrafish, disrupting s1pr2 (S1P receptor) or spns2 (S1P transporter) results in defective migration of these cardiac progenitors and in cardia bifida (two separated hearts), indicating S1P signaling regulates myocardial migration (Kupperman et al., 2000; Osborne et al., 2008; Kawahara et al., 2009).
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We downloaded ESBs from the ECRBase database (Ovcharenko et al. 2005b) and evaluated the breakpoint density in the regions separating heart enhancers and their target genes.
For example, the six frequent common conditions approach bundles all diagnoses related to heart disease into a single category, while the FCI separates heart disease into three categories; angina, congestive heart failure (CHF /heart disease, and heart attack.
In a particularly thankless role, Paul Fitzgerald is surprisingly touching as Nick, a newly separated lonely-heart desperately seeking a much younger partner.
After undergoing two separate heart procedures since leaving office a decade ago, former President Bill Clinton changed his diet to slow the progression of heart disease.
At St. David's Medical Center in Austin, where he went for two separate heart procedures last year, the hospital's admitting office looked at Mr. Yurdin's coverage and talked to Aetna.
Because it doesn't require a separate heart rate band or battery-hungry pulse sensor, it can grab a more interesting set of data.
Instead there are many separate heart ailments, or combinations of them.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com