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Upon the completion of the evaporation step the samples were separated employing a silica-gel chromatography column with a 100-ml mixture of petroleum ether and diethyl ether with a volume ratio of 30/70.
The bacteria and blood cells were separated employing their different DEP behaviors, and furthermore, the concentrated bacteria produced an amplified positive DEP force for adsorption of AgNPs on the bacteria surface.
Nuclear proteins (17 μg) were separated employing 10% bis-Tris NuPAGE gels (Invitrogen), transferred to nitrocellulose membranes and probed with an anti-CO antibody followed by a horseradish peroxidase-conjugated secondary antibody.
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The compounds of the concentrated solution were separated by employing a hydrophobic silica column with a SepacoreX50 (40 63 µm particle size, 60 Å pore size, 150 mm column length, 12 mm column diameter; Büchi Labortechnik GmbH, Flawil, Switzerland).
Another approach would be to consider restricted analyses that separate employed and unemployed study participants.
The supernatant of the homogenate was separated and employed to estimate the total protein content and the thio-barbituric acid reactive substances.
Based on their characteristics of phase equilibria and separating agents employed, a classification of adsorption processes has been proposed as 'absorption like'distillationion like' and 'chromatographic' processes, which provides insight into these processes and a rational basis for their design.
However, the extent to which domain-specific metacognitive processes can be separated in studies employing correlative techniques is unclear.
When opinion pieces are included, the Times has published at least 15 separate articles employing such language, depicting Chávez as a "dictator" or "strongman".
The subsequent investigation revealed breaches of Health and Safety legislation and in 2007 the Health and Safety Executive issued a press release making an example of the 2004 incident in which the separate company employing the man, and Mansel Raymond, were prosecuted.
Stone sculpture occurs in several separate centres, employing both hard and soft rock, but there is usually not much evidence of a development through time in a single place.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com