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To determine the purity of separated cells, separated cells were centrifuged at 300 × g for 5 min and washed twice with RPMI-1640 medium.
Additionally, interconnected graphitic carbon walls, forming separated cells, can ensure high electrical conductivity, which were formed after acid-leaching of metallic components.
The conceptual idea is to convert traditional linear raceways into a series of hydraulically separated cells, each of which behaves as an individual circular tank.
In addition, cell fates become fixed at the first cleavage of the early embryo, so separated cells cannot form twins. Larval (immature) forms, if present, are called trochophores.
The largest electric field intensity (∼10 kV/m) was observed at the edges of the microelectrodes, in the close proximity of magnetically separated cells, which is optimal for subsequent cell electroporation.
Integration of specially designed membrane modules with traditional fermenter culminated in development of a hybrid process with high process intensification that permitted fermentation under high cell density with recycle of separated cells and unconverted sugars.
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The distance vector x b − x a between two particles residing in two well-separated cells A and B, respectively, can be decomposed into three components (see also Figure 14) x b − x a = r + r b − r a (25).
To avoid cytotoxic activity due to non-matched cells the CD14-negative cultures were generated from the same donor as the non-separated cells.
The mean relative growth index per day was higher in FACS-separated cells and lower in MACS-separated cells in P2, resulting in a significantly higher proliferation of FACS vs. MACS-sorted cells.
The significantly modified Sf and Par tissues by DAP produced a heterogeneous substrate of semi-separated cells.
Total RNA was extracted from MACS-separated cells using the RNeasy Micro Kit (Qiagen, Valencia, CA) following the manufacturer's protocol.
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