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To distinguish the different potential for cell proliferation, we separated cell populations into quiescent and active cells.
Because DNA was not available for individuals I∶1 or I∶2, transmitted haplotypes were arbitrarily assigned to the parental generation by genotyping chromosome separated cell lines from a single affected individual in generation II.
To test this possibility, we screened various culture substrates, and unexpectedly found that H1 cells grown on non-tissue culture treated (NTC) plates in CM with the Rock inhibitor were able to maintain individually separated cell growth at the same concentration as that used for mES cells while maintaining high nuclear-cytoplasmic ratio (Figure 5A and B).
Hierarchical clustering separated cell lines from tissue but did not provide information on how well cell lines model cervical cancer.
To provide an alternative approach to determine complex formation, we separated cell lysates from the QSOX1A V5-expressing cell line by SEC.
Total RNA was isolated from 2 × 106 cells (PBMC, SFMC or separated cell fractions as indicated) using RNAzol (Biogenesis, Poole, UK) according to the manufactuer's instructions.
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To keep the TICs separated, cells were taken up in cell dissociation solution (Sigma-Aldrich).
Samples were centrifuged (10 min, 10,000 rpm) to separate cell mass and the supernatant.
The bilayer architecture of the microchip separates cell handling and positioning from cell activation by soluble chemicals.
This analysis revealed two distinct groups, separating cell lines according to synergistic and antagonistic behavior.
TFE3 phosphorylation was also examined in MEFs by separating cell lysates through 7.5% SDS PAGE.
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