Regardless of their origin, they were shown to form various sized aggregates with full-length CF40.Gly.NS3pro that can be separated by size exclusion chromatography, yielding fractions of active protease of sufficient purity for crystallisation trials.
A staff of six ensures that the dogs, which are separated by size, are under constant supervision.
In addition to being separated by size, proteins can also be separated according to their specific charge residues.
Starch granules were separated by size using a dextran solution flow (250 g/L).
The POM was separated by size fractionation (> 53 μm) from coarsely sieved (> 6 mm) soil.
Additionally, traditional capillary electrophoresis (CE) assay design further inherently limits shortening amplicons because the markers must be separated by size.
Protein aliquots (30 μg) were separated by size on a 10% SDS-tricine-polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Novex/Invitrogen, San Diego, CA).
The cleaved AcrIIA6 protein was eluted from HisTrap FF crude Ni2+-chelating column and was further separated by size exclusion chromatography (Hiload 26/600 Superdex 75 prep grade, GE Healthcare) with 20 mM Hepes pH 7.5, 150 mM NaCl.
spectroscopy and static light scattering (SLS), molecular weight distribution (MWD) and chemical composition distribution (CCD) were investigated for as-polymerized samples and their fractions separated by size exclusion.
Later, the method was performed by using automated sequencing machines, in which the truncated DNA molecules, labeled with fluorescent tags, were separated by size within thin glass capillaries and detected by laser excitation.
We have developed an assay where labelled sample proteins are separated by size exclusion chromatography (SEC) before contact with microsphere-based arrays (Size-MAP; size exclusion chromatography-resolved microsphere-based affinity proteomics).
