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In this method, lipoproteins separated by permeation columns (exclusion chromatography), the lipid components (mainly cholesterol and triglycerides) are detected enzymatically.
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They are also excluded by the gel permeation resins and separated by the ion exchange resins used in the chromatography processes.
The concept is like a combined counter/cross-flow parallel-plate membrane contactor, where the refrigerant (water) and the absorbent (salt solution) flows are separated by the semi-permeable membranes, which only guarantee the permeation of water vapor.
During OSN, solutes are separated by size exclusion and geometric selection as they pass through a membrane possessing nanometer-scale permeation pathways.
Supernatant and cells were separated by centrifugation.
Monocytes were separated by two different methods.
The strong electrostatic effect on cGMP binding and absence of effect on ion permeation through open channels implies that the cGMP binding sites on the channel are well separated from the permeation pathway.
The Maxwell Stefan theory is used to demonstrate the possibility of separating the hydrocarbon isomers by permeation across a silicalite membrane.
The model separates the permeation steps and consequently their influence, quantifying the relative resistances offered by each of them.
To investigate this hypothesis, we released cell wall associated carbohydrates in CPS-C (E. faecalis FA2-2 and E. faecalis type 2) and CPS-D (E. faecalis type 5) strains by enzymatic digestion of peptidoglycan and separated the extracted material by gel-permeation chromatography.
The relative amounts of amylose and amylopectin in starch granules were compared between wild type and the three mutant lines after separating starch polymers by gel permeation chromatography (GPC).
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