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Proteins were separated alongside a molecular weight marker (BioRad, Hercules, CA, USA).
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Purified proteins were separated in gels alongside ultrapure BSA standards (Biorad), and coomassie-stained bands were compared to determine the amount of each protein isolated.
Biotin-labeled proteins were separated by SDS-PAGE alongside total cell proteins.
cDNAs are radioactively labelled and separated by gel electrophoresis alongside a sequencing ladder.
27 Participants performed three 15 m sprints as fast as possible separated by 15 s alongside the outdoor tennis court.
Aliquots of soluble protein samples equivalent to 12 μg proteins were separated on SDS-PAGE alongside 60 ng of the native ScAGU115 α-glucuronidase [ 40].
For analysis of tau isoforms, samples were dephosphorylated prior to electrophoresis using lambda protein phosphatase as described previously (46) and separated by SDS-PAGE alongside a recombinant tau ladder (Sigma).
Briefly, proteins were separated as described above alongside Odyssey pre-stained molecular weight markers (Licor, fluorescence in 700 nm channel) and transferred onto PVDF membrane (Millipore) by wet blotting.
During Wednesday's practice round, I walked up the fairway directly alongside VJ Singh separated only by a thin rope.
Amplified fragments were separated on a 1% agarose gel alongside a DNA ladder of 100-bp increments, which was included to confirm fragment molecular weights.
Our camans were separated by 100 years and seeing mine placed alongside William's was something very special.
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