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For example, the alternative splicing of Fibulin-1 transcripts results in four separate variants termed -1A to -1D, which differ at the C-terminus of the protein.
A GFP variant containing 11 Phe residues was used as starting scaffold to generate 10 separate variants in which each Phe was replaced individually (in one construct two adjacent Phe residues were changed simultaneously), while retaining varying levels of activity.
In addition, no significant difference could be found between the cases and controls for any of the four separate variants (Table 5).
The incursion of 2 separate variants into northern India and Nepal might be a consequence of difficult access between these 2 areas due to the regional terrain.
Typically, they also assign each variant a score, based on analysis of protein structure or evolutionary conservation, to separate variants with little functional impact from those more likely to damage protein function (83, 84).
RT-PCR alone would only be considered reliable for confirmation of homozygous mutations (and in one case, a compound heterozygote where two separate variants abolished natural splicing of the same exon), unless combined with a secondary quantitative methodology.
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However, another major reason for rising costs was the need to develop a separate variant for the photo-reconnaissance mission when the underwing pods caused too great a performance penalty.
As a separate variant, the C70U change alone, Table 1, is also unstable in the E. coli cellular context.
Thus, there is evidence for three independent variants in SLC2A9 that influence urate levels in Europeans and for a separate variant in East Asians.
For example, if a GWAS analysis deals sequentially with one million separate variant-disease associations, it is perfectly acceptable to model one of those associations in isolation.
It loads three separate Variant Call Format (VCF) files, and sample VCF files were generated using the GATK Unified Genotyper (DePristo et al., 2011).
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