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The resulting extract was divided into five fractions: two for analysis by two separate reverse phase (RP /UPLC-MS/MS methods with positive ion mode electRP /UPLC-MS/MStion (ESI), one for analysis by RP/UPLC-methodsith negative ion mode ESI, one for analysis by hydrophilic interaction chromatography (HILIC)/UPLC-MS/MS with negative ion mode ESI, and one sampositivereserved for backup.
Another was the ability to go astern at full power without needing a separate reverse turbine to do so, simply by reversing the electrical polarity of the motors.
We carried out four biological replicates with four separate RNA pools and four separate reverse transcription reactions for ovary experiments.
For the two-step protocol, 2 separate reverse transcriptions were performed, one for the 5' parts and one for the 3' parts of the segments.
A separate reverse transcription was perfomed for endogenous control (18S ribosomal RNA) using the SuperScript III First-Strand Synthesis System (Invitrogen, USA).
Three separate reverse transcription reactions per sample were performed.
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18, 19 Briefly, three separate reverse-transcription PCRs were performed which overlapped to cover the core to nonstructural (NS) 5B region.
Three separate RNA reverse transcriptions and three amplification reactions were performed per sample to interrogate all 481 T-UCRs.
Indeed, all the items in the Skin Authority Man line, including a daily sunscreen and a toner, are labeled "age-defying," an acknowledgment that most men are not likely to buy a separate wrinkle reverser.
Digests were separated on reverse phase C-18 HPLC resin using an acetonitrile gradient in the presence of TFA.
Subsequently, derivatives were separated by reverse phase HPLC.
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