Sentence examples for separate restrictions from inspiring English sources

For example, I have two separate restrictions, from two different branches of the security forces, that forbid me from leaving Syria.

Despite a warning by the United States trade representative, Robert B. Zoellick, that a Congressional roadblock "violates the spirit of Nafta by holding Mexico to different standards than it holds Canada and the United States," the House and Senate passed separate restrictions.

In a separate restriction, one that a Palestinian consultant said would be seen as a slap at the Palestinian leadership, the spending measure effectively bars the money from going to the Palestinian Authority itself or its president, Mahmoud Abbas, who is known as Abu Mazen.

Electrophoresis to separate restriction fragments was done using 2% agarose gels in a TAE buffer system (20 mM Tris, 10 mM acetic acid and 0.5 mM EDTA; pH 8) at 80 V.

Three separate restriction fragments of the genomic DNA of PPV were cloned into the pSU18 plasmid.

In addition, there are 14 separate restriction enzymes and 18 unaccompanied DNA methyltransferases.

Gel electrophoresis is used to size separate restriction-digested DNA, which is then transferred (or blotted) to a solid support such as a filter for probing and detection by radioactive or luminescent labelling.

The resulting 291 bp fragment was cloned into the BglII/ XhoI sites of a pcDNATM 6.2GW/EmGFP-miRNA-1.2 from a separate restriction enzyme digest thereby generating pcDNATM 6.2GW/EmGFP-miRNA-1.4 (contains 4 tandem copies of miRNA-1).

The resulting 152 bp fragment was cloned into the BglII/ XhoI sites of a pcDNATM 6.2GW/EmGFP-miRNA-1 from a separate restriction enzyme digest generating pcDNATM 6.2GW/EmGFP-miRNA-1.2 (contains 2 tandem copies of miRNA-1).

We compared in silico digests of the BAC sequences by three separate restriction endonucleases (BamH1, EcoR1, and HindIII) to the same generated in the laboratory and imaged on a high resolution gel electropherogram.

Briefly, we amplified a short PCR fragment containing a diagnostic SNP (eyeF, 5′-TGT GTG AGC AAA ATT CTC GG-3′; eyeR, 5′-GTT TCG GCA TGG TAG GAC AT-3′), digested with MboII, and genotyped by separating restriction fragments on a 2.5% agarose gel.