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Each lane in the immunoblot is from a separate mouse.
The scientists are now checking in separate mouse experiments whether the same is true for the tau protein.
Sure, I miss the comforts of my office – the big monitor and separate mouse, the reliable Internet connection – but these are just luxuries, really.
In one project, Microsoft's programmers developed software that added multiple cursors on the screen, each controlled by a separate mouse.
Interestingly, when Chmp7 expression was examined in the spinal cord of presymptomatic mice from two separate mouse models of MND, the SOD1-G93A model mofel of Amyotrophic Lateral Sclerosis (ALS) and the SETX-R2136H mofel of ALS4, an adolescent-onset form of ALS, we found no significant differences between WT and mutant mice (Supplementary Information, Fig. S5).
We used separate mouse models of acute myocardial ischemia-reperfusion injury and renal ischemia-reperfusion injury.
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Separate mice (n=6) were killed immediately after NOD testing by the same method to provide brains for Cd11b immunohistochemistry, or by concussion and immediate cervical dislocation (n=16) to provide dorsal hippocampi for qRT-PCR and western blot analysis of VGLUT1 mRNA and protein expression at the same postsurgery timepoint where key cognitive deficits were identified.
In each model, separate mice were sacrificed in a time sequence.
Results are presented as mean ± SD of four separate mice in one group.
For kidney I/R injury in separate mice, following midline laparotomy, renal arterial blood flow was interrupted for 1 h followed by reperfusion.
This focus on the evolutionary extremes of apes and mice, however, leaves unexamined many important questions raised by the more than 90 million years of evolutionary change that separate mice and men.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com