Genomic DNA (5 μg) was separated into fragments of ∼6 kb by g-TUBE (Covaris) centrifugation and purified using a QIAquick PCR Purification Kit (Qiagen).
The Golgi network, normally located near the nucleus, is disrupted and separates into fragments in cells expressing marshalin-Ld (Fig. 7A, arrowheads).
It has been shown that with carefully selected cross-flow programs, focusing times, and membrane materials, samples of VLPs can be separated into fragments, monomers, dimers, oligomers, and aggregates of VLPs (see Fig. 6).
Colonies of the free-living bryozoan Cupuladria exfragminis [Herrera-Cubilla, A., Dick, M.H., Sanner, J., Jackson, J.B.C., 2006. Neogene Cupuladriidae of Tropical America. I:. Taxonomy of Recent Cupuladria from opposite sides of the Isthmus of Panama. Journal of Paleontology. 80, 245 263.] from the Gulf of Panama are observed to separate into viable fragments without external force.
In the anole lizard the single copy lysozyme g gene is separated into 5′ fragment and 3′ fragments (Addition file 1: Table S1), but an intact coding sequence (supported by EST sequences such as accession number FG795243.1) spanning these segments was found in the NCBI database (Additional file 2: Table S2).
Most of what was found is separated into 15,000 fragments that make up about 900 documents, fueling a longstanding debate on how to order the fragments as well as the origin and meaning of what is written on them.
The cell elongated and separated into two fragments, the larger one containing the nucleus.
Gene clusters that are often conserved among chromist plastid genomes [25] are lacking in the K. veneficum plastid genome (Fig. 3A), including the atpA-operon and the well-known ribosomal protein superoperon, which is highly reduced and separated into five fragments (Fig. 3B).
For RNA preparation, the trabecular bone samples were rinsed briefly in diethylpyrocarbonate-treated water (Sigma-Aldrich Pty Ltd, Castle Hill, NSW, Australia) and then separated into small fragments, using bone cutters.
In case of the product having the G428A mutation, the 107 bp product might be separated into two fragments, 23 and 84 bp, by XbaI digestion.
For total RNA extraction, the trabecular bone samples were rinsed briefly in diethylpyrocarbonate-treated water and then separated into small fragments, containing bone and bone marrow, using bone cutters.
