Two arteries were pooled for each sample, and densitometry reflects 12 14 samples per group on five separate blots.
Samples were resolved by SDS-PAGE with 15% polyacrylamide gels, and separate blots were run for each protein.
and β-actin (Clone C4, MP Biomedicals, LLP, Ohio) were probed on separate blots, as all these proteins are of similar sizes.
The results of Western blotting for phosphorylated STAT3 for these samples were from two separate blots.
Two separate blots were hybridized with ATP synthase (mitochondrial enzyme) and cytoplasmic protein GAPDH.
Separate blots were used to probe for phosphorylated and total STAT1.
Both CBC8C (1 100) and anti-TCTEX1D4N (1:1000) antibodies were used in separate blots for immunodetection of protein expression.
For each sample, the average of two mean TLs calculated from two separate blots was used for statistical analysis.
Densitometry measurements were done using Quantity One software (Bio-Rad Laboratories, HerCAles, CA, USA) and results were averaged from 2-3 separate blots.
Blots were blocked in PBS-Tween and incubated in 1 μg/ml rhPln.198 or rhPln.247 (separate blots) in PBS-Tween overnight at 4°C with rocking.
