Rhagoletis genomic sequence reads that were identified as similar to one of the D. melanogaster type I prenyl protease loci in the four separate tblastx searches were separately assembled using Sequencher (Gene Codes Corp).
To evaluate, we performed "separate assembly" by assembling PE reads from G4 and S4 into scaffolds separately, and SE reads from other 10 samples into contigs by using SOAPdenovo [25], [26], and then combined the three parts by using CAP3 [27]; we also performed "pooled assembly" by assembling the pool of all reads using SOAPdenovo, followed by using CAP3 as well.
Our simulations thus confirmed that it is possible to assemble short reads from this type of mixture of mitochondrial genomes and to separate the assembled contigs into the individual components of the mixture.
After the initial separate assembly, the contigs assembled and the remaining unassembled sequences were subjected to a secondary assembly using MIRA.
Two separate towers were assembled for television cameras.
This photo of the Andromeda galaxy is the largest composite (made from 7398 separate exposures) ever assembled from the Hubble telescope.
We added in quadrature when propagating uncertainties within a livestock type, because we independently assembled separate estimates of the various carbon fluxes and their uncertainties (e.g. intake, manure production, milk production, CH4 emissions) except for CO2, which is calculated by subtraction.
To test the influence of spacer length and composition, separate reactions were assembled using 5' radiolabeled dCHr8 substrate in combination with all sixteen bridge oligonucleotides: HEG-G, AnG and TnG (n = 0, 1, 2, 3, 4, 6, 8, and 10).
Separate transcriptomes were assembled for each genotype.
Both pieces were fabricated through separate steps and assembled forward electrode modification with laccase.
