In a separate analysis, we applied methods to distinguish the impact of a causal factor through an intermediate versus a direct path (Petersen et al. 2006).
Compared to the separate analysis -although both methods give complete molecular information on biological processes related to pigmentation during development, pigmentation, melanocyte differeniation and pigment cell differentiation- the joint analysis enriches the description of Melanogenesis, that is the major process upstream the melanocyte differentiation and pigmentation.
Barriers included those intrinsic to research methods, such as differing time lines of methods leading to separate analysis and publication; those related to the skills and preferences of researchers, such as different team members undertaking different components of a study; and structural barriers, such as different journals emphasising either qualitative or quantitative research.
Comparison of the annotations done on the joint versus the separate analysis for the FA based method (indicated with FA).
In that context, most of the existing strategies involve the ANOVA-based partitioning of sources of variation and the separate analysis of ANOVA submatrices using multivariate methods, to account for both the intrinsic characteristics of the data and the study design.
Because Cr VI) is reduced to Cr III) both intracellularly and extracellularly and because analytical methods for the separate analysis of Cr VI) or Cr III) in biological samples are not available, the speciation of the tissue chromium after exposure to Cr VI) was inferred by comparing total chromium concentrations in tissues of rats and mice exposed to similar doses of Cr VI) or Cr III).
A number of exploratory data analysis methods are available in separate panels, including alpha diversity estimates, multivariate ordination methods, as well as network, heatmap, scatter and bar graphics.
We compared our FA-based approach with other methods in two ways: i) joint analysis versus separate analysis and ii) FA-based joint approach versus other joint method.
Notably, no correlation or agreement between the two analysis methods was found when separating the values for baseline and shock.
Advanced time series analysis methods have been applied to separate different process effects in the multiple signal matrix.
