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Fifteen colonies with the most absorbance at 450 nm were sent for sequencing.
A renewed PCR reaction was performed for representative specimens to be sent for sequencing.
The amplified PCR products were sent for sequencing to Yaazh Xenomics, Chennai, India.
The PCR-amplified fragment was purified with DNA purification kit (Thermo Fisher Scientific, Canada) and sent for sequencing.
The amplified PCR product was purified using illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare Life Sciences) and subsequently sent for sequencing.
PCR products were sent for sequencing analysis to confirm results.
The plasposon was purified from cultures of recombinant cells and sent for sequencing to QIAGEN.
PCR products were sent for sequencing to Genaissance pharmaceuticals (Connecticut, USA).
Periplasmic extracts containing VHH were prepared according to standard protocols and selected VHH were sent for sequencing.
For the TrpC3, BNP, and GAPDH primer sets, the PCR products were also sent for sequencing to confirm that the proper target had been amplified.
15 μg DNA were sent for sequencing.
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