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He sent for both companies' catalogues and studied the available titles.
Liver biopsies should be sent for both microbiological and histological evaluation.
In addition, patients provided an early-morning sputum sample on the morning following admission; this sample was sent for both direct and concentrated smear microscopy (as described below).
The total number of case notes (1260) is slightly different to the number in the explicit review because not all notes had been sent for both types of review when the study "closed".
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In addition, the PCR products were sent for sequencing in both directions (Bioneer, Korea).
Following PCR, amplicons were purified with the CONCERT™ Rapid PCR purification system (Life Technologies, USA) and sent for sequencing on both strands to Macrogen Inc (Seoul, Korea), with the same primers used for PCR.
A system was in place to collect and record all the results of specimens sent for TB culture (both positive and negative), but it is also possible that some may have been missed.
The willow PCR products were cleaned with 1 μl of a mixture of Exonuclease I (New England BioLabs) and Shrimp Alkaline Phosphatase (SAP) (Fermentas) for every 5 μl of PCR product before they were sent for sequencing of both strands with the forward and reverse PCR primers.
Attempts were made to characterise mutations on at least two different occasions, forming part of two subsequent biological repetitions; the amplification products were purified by using a Promega Wizard purification systems kit and then sent for sequencing in both directions (5′ and 3′) with a BigDye Terminator kit (Macrogen, Seoul, South Korea).
The specimens obtained during OLB were sent for tissue culturing (for both bacteria and viruses); all culture results were negative.
When Mary Godwin gave birth to a daughter in February 1815, she immediately sent for Imlay, particularly as both she and the infant were ill.
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