The mice were divided into untreated (normal; 200 μL of 70% EtOH/mouse/day), D. farinae-sensitized (control; 70% EtOH), D. farinae-sensitized plus Protopic® ointment-treated (Protopic; 50 mg/mouse/day) and D. farinae-sensitized plus AC extract-treated (AC; 10 mg/mouse/day) groups.
A separate case control study in Palestine that included 6 to 12 year old children [ 25] showed inverse associations between medium endotoxin levels from the mattress and being a non-sensitized case (report of wheeze in the past 12 months) compared to a non-sensitized control [ 25], in agreement with the results of our study.
No mitochondrial abnormalities were detected in non-sensitized controls or in gluten-sensitized mice without indomethacin (Figure 2A,B).
HRP flux, a measurement of transcellular macromolecular transport, was elevated in all groups compared to non-sensitized controls.
Gluten sensitized mice and non-sensitized mice treated with indomethacin exhibited a mild retardation of weight gain after 7 weeks compared to non-sensitized controls.
While IgE-sensitized controls would have made an ideal reference group, their limited size (n = 23, 11%) did not allow comparison with the sensitized cases (n = 92, 46%).
Gluten-sensitized mice treated with indomethacin exhibited a significant increase in small intestinal tissue conductance compared to non-sensitized controls and indomethacin alone-treated mice (Figure 1A).
Increased T cell proliferation after incubation with PT-gliadin was observed in gluten-sensitized mice, but not in non-sensitized controls (Figure 5).
Gluten sensitization and indomethacin treatment, however, led to the highest increase in HRP flux with approximately 2.5 fold increase compared non-sensitized controls (Figure 1B).
Gluten-sensitized and indomethacin alone-treated mice showed reduced E-cadherin RNA expression by a mean factor of 0.762 and 0.533 respectively, but this did not achieve statistical difference relative to non-sensitized controls (Figure 4).
