Next, we examined the possible functions of the nuclear anchoring in the mom-2/Wnt (null) sensitized background, because mom-2 null) mom-2 nullre not comutantsy defective in asymmetric cell fate determination (83% defective n = 30) and POP-1 asymmetry (Fig. 5d).
In contrast to the dramatic effect seen for the plasma membrane-resident ZnTs, none of the multiple RNAi lines for the whole set of ZnTs located in different parts of this pathway displayed any zinc absorption defect, and this was the case even in an sensitized background (dZnT1 RNAi) and using the sensitive ALP activity assay.
Of note, S. bayanus is more sensitive to copper than the laboratory strain of S. cerevisiae; our screen in the sensitized background of S. bayanus likely provided added sensitivity to detect genes involved in the response to copper.
Also, the sensitized background of e1368 revealed that F58D2.1 does in fact encode a hypoxic sensitivity promoting activity that was not apparent in a wild type background.
Second, the gain-of-function design of pFind1 enabled us to perform a genetic screen for reprogramming factors in a sensitized background.
Using this neurodegenerative phenotype as a sensitized background for a genetic modifier screen, we have identified mutations in four genes: staufen, muscleblind, split ends, and CG3249.
RNAi was performed on a sensitized background strain, OH9315 (eri-1; lin-15b; vtIs1).
All three compounds caused a dose-dependent neuroprotection in the pqe-1 sensitized background (Figure 2B).
We predict that this threshold would be lower in a Wg/Wnt sensitized background.
In absence of a homozygous phenotype we made use of the dominant suppressor phenotype in our sensitized background.
In this genetically sensitized background, the 103 position that gets phosphorylated in the wild type protein becomes decisive for the function of the entire protein.
