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The checkpoint protein Rad9, like its mammalian ortholog 53BP1, inhibits ssDNA accumulation and was used to sensitize some strains to the accumulation of ssDNA (Lazzaro et al., 2008; Bunting et al., 2010).
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Hydroxychloroquine (HCQ) inhibits autophagy and sensitize some cancer cells to chemotherapy48.
Because of the established role of capsule in resistance by E. coli to serum killing, chemical capsule inhibition would be expected to sensitize encapsulated strains to serum exposure, thus attenuating infection and providing a useful therapeutic intervention.
Enforced expression of Bcl-xL sensitized some cell types, but not others, to ABT-737.
However, in absence of other supporting evidence, genetic interaction most likely reflects a broad role for the splicing factors in maintaining efficient RNA regulation rather than specific contribution to the function of the gene mutated to sensitize a strain to genetic interaction.
The radC strain was normal for host cell reactivation of gamma- and UV-irradiated bacteriophage lambda; the radC mutation did not sensitize a recA strain, but did sensitize radA and polA strains to X and UV radiation, and a uvrA strain to UV radiation.
In contrast, a deoR knock-out sensitized this strain to the drug, resulting in 97% killing after 3 hours.
Since the recN2001 radB) mutation sensitizes a uvrB strain to UV irradiation, this suggests strongly that it must play a role in postreplication repair.
(7) It had been reported that a deficiency in DNA polymerase I (polA) sensitized a uvrA strain of E. coli to UV irradiation.
Compounds that sensitized the K12 K1 strain to T7 phage were deemed to affect capsule, and 2 of 9 compounds rendered EV36 sensitive to T7.
We next examined whether the two useful backgrounds above might be combined to create a doubly sensitized strain.
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