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However, in absence of other supporting evidence, genetic interaction most likely reflects a broad role for the splicing factors in maintaining efficient RNA regulation rather than specific contribution to the function of the gene mutated to sensitize a strain to genetic interaction.
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Even one or two bottles of a cow's milk formula may be enough to sensitize a newborn infant and cause an allergic reaction.
Similarly, we also found that the same rpn1 alleles further sensitized an rpn13-KKD rpn10-uim strain to AZC.
Real experience working in startups sensitizes a board member to these realities of startup life.
First, depression as a precursor for pain, sensitizing a person to experience pain [ 7].
Because of the established role of capsule in resistance by E. coli to serum killing, chemical capsule inhibition would be expected to sensitize encapsulated strains to serum exposure, thus attenuating infection and providing a useful therapeutic intervention.
The checkpoint protein Rad9, like its mammalian ortholog 53BP1, inhibits ssDNA accumulation and was used to sensitize some strains to the accumulation of ssDNA (Lazzaro et al., 2008; Bunting et al., 2010).
In contrast, a deoR knock-out sensitized this strain to the drug, resulting in 97% killing after 3 hours.
We next examined whether the two useful backgrounds above might be combined to create a doubly sensitized strain.
Compounds that sensitized the K12 K1 strain to T7 phage were deemed to affect capsule, and 2 of 9 compounds rendered EV36 sensitive to T7.
Thus, we mounted a screen of 2,080 previously approved drugs (Supplementary Table 2) for compounds capable of potentiating the activity of cefuroxime, a broad spectrum β-lactam that was highly sensitized in the ΔtarO strain, against the clinically relevant CA-MRSA strain USA300 (Supplementary Figure 3a).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com