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For the sensitization experiment WT, and NR1DATCre mice were administered four pairs of injections (one intracranial and one i.p ., with one pair given everyday.
For example, PVT lesions blocked the conditioned locomotor response to a cocaine-paired environment in a sensitization experiment [30], whilst PVT activation has been shown to be associated with increased locomotor responses entrained to regulated feeding schedules [31].
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For sensitization experiments, olfactory stimulus duration was 500 ms and each pulse was separated by 9.5 s.
The elevation was greater in the cocaine HCl-trained animals, consistent with what is seen in cocaine sensitization experiments [38], [39], [40].
For the sensitization experiments, quarter turn data (in either direction, totaled over 90 min) taken during the five chronic treatment sessions were analyzed using 2-way ANOVA with METH history (naïve, METH-treated) as a between-subjects factor and day (1, 3, 5, 7 or 9) as a within-subjects factor.
To establish the minimal irritating and maximal nonirritating concentrations of the CPE for use in sensitization experiments and to ensure that the mice would tolerate multiple days of topical exposure to the ear, we conducted a primary irritancy assay following a modification of the method described by Hayes and Meade (1999).
For Ca2+-sensitization experiments, myograph-mounted arteries were permeabilized with α-toxin and Ca2+-clamped with 10 mmol/L EGTA, as described previously.
To avoid sensitization, the experiments were planned so that repeated injections were administered with more than 8 weeks interval and a maximum of 4 tests were performed for each animal.
Consistent with time-dependent sensitization, behavioral experiments are generally undertaken 1 ~ 14 days after the SPS procedure [ 23].
Design of the cisplatin sensitization HT screening experiment The model is the same for all siRNAs and hence we drop the siRNA index when not referring to a specific siRNA.
On the basis of these experiments, sensitization to TRAIL-induced apoptosis was observed in all the tested myeloid leukemia cell lines.
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