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At 2 years of age, all children were assessed for atopic sensitization, AD, asthma, and allergic rhinoconjunctivitis.
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For sensitization, 50 mg Biostir-AD was topically applied on the upper dorsal skin and the back of the ears twice weekly for 4 weeks.
It is striking that the proportion of children with AD and allergic sensitization such as in the study of Taylor [ 49] and Huurre et al. [ 97] was significantly higher in the probiotic group.
Importantly, we show the anti-inflammatory activities of these repositioned drugs translated to an in vivo epicutaneous sensitization mouse model of atopic dermatitis (AD), an inflammatory skin disease.
113 In a literature review, Flohr et al estimated that only a third of patients with AD show true IgE sensitization.
13 Defects of epidermal differentiation such as altered filaggrin expression weaken the epidermal barrier and predispose patients with AD to epicutaneous allergic sensitization as well as to physical, microbial and irritant skin damage.
However, 40%60%% of children with AD may not develop IgE sensitization [ 10].
This finding together with the fact that about 20 40% of AD patients display intrinsic AD with no sensitization to any protein allergen suggests the importance of microbes (11).
In Taylor et al.'s trial, probiotic supplementation postnatally failed to reduce the risk of AD and increased the risk of allergen sensitization in high-risk children.
AD-like skin lesions were induced by sensitization to and repeated challenges with picrylchloride in the Th2-skewed NC/Nga mice strains.
AD-like skin lesions were induced by sensitization to and repeated challenges with picryl chloride in the Th2-skewed NC/Nga mouse strain.
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