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Dudley et al. [ 36] compared the sensitivity of the methods, and GS/MS was shown to be tenfold less sensitive than HPLC/IT-MS in the analysis of nucleosides.
The sensitivity of the methods was estimated by analyzing the reference DNA fragment containing 5hmC provided by the kit.
However, a rough comparison of the sensitivity of the methods can be made if these aspects are kept in mind.
The method was designed in order to compare the sensitivity of the methods that use quercetin and the sulfonic derivative.
The level of detection of a transfer event according to the sensitivity of the methods used was 10−6.
These results are indicative of the sensitivity of the methods to experimental conditions and demonstrate the power and the statistical limitations of digital expression analyses.
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Toward this end, we further evaluated the sensitivity of the method under inflammatory conditions.
The sensitivity of the method permitted detection of ~3 fmol DA.
The sensitivity of the method was 3.7 × 102 μA mM−1.
An important part of the experimental setup was the analysis of the detection sensitivity of the method to the data used for training.
All reported interfaces had significantly more support than this strict requirement and the on average 13.6 identified cross-links per interface demonstrates the sensitivity of the method.
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