Furthermore, the noise in marker gene expression measure decreased the sensitivity of PSEA (see Methods).
The Bioline Optima ELISA method showed the poorest performance of all methods included in the study, with an overall calculated sensitivity of 0.23 (see Table 1).
> The low sensitivities of MRL seen in the early study by Keller et al. may have been due to low patient numbers and total nodal metastases.
A score of −1.167 (fold change 0.833) or greater for miR-145 had 93.3% specificity but had sensitivity of only 93.8% (See Figure 4, Table 4).
Finally, we plot the false positive rate (1−specificity) versus true positive rate (sensitivity) of a test (see Figure 3) for individual miRNA's ROC analysis.
We thus compared the two tasks also in terms of the distance metrics d' (Figure 3D F), which estimates the system sensitivity to sensory signals independently of response criterion, and bias, which estimates the response criterion independently of sensitivity (see Methods).
The loss of reproducibility and decrease of Sensitivity were seen also in case 10 % and 5 changes (Supplemental Figure S2A, C) for both SAM and Limma methods.
Notably, different levels of sensitivity seen in six other mutant variants A231G, V364A, M132A, P512A, S505A and V496A points to the fact that even though there is a single pore for proton antiport and drug binding and translocation, yet there are residues which impart selectivity in drug recognition along with those which affect the transport of all the drugs.
Accordingly, the area of sensitivity is estimated to be a sphere with diameter twice the electrode surface diameter, i.e. a 200-μm-diameter electrode has ∼400-μm-diameter sphere of sensitivity (see also Thompson et al., 2003).
This was not compatible with the elevated topo II activity (see Figure 2), and the sensitivity of mammosphere-derived cells (see Figure 8).
