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If enough WBCs (2.0 × 10) could be reached, the sensitivity of methodology of eight-color flow cytometry could be as high as close to 1.0 × 10−5.
Although we do not understand the basis for these differences, it might be attributable to the editing tools used, differences in ratios of donor versus nuclease-expressing vector used, and/or sensitivity of methodology used to score the genome editing events.
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Although this was a preliminary try, the study initially tried to define the difference of lipidomic profiles between cancer subgroups which is a great challenge due to the significance of limited difference between subtypes, the variation of lipid species, or the sensitivity of methodologies.
The purpose of this study was twofold: to develop a methodology for the estimation of land surface temperature for non-urban areas and to analyze the sensitivity of the methodology.
As expected, donor CD4+ and CD8+ T cells had reduced IL-7Rα expression when transferred into Rag2KO but not untreated naive recipients (Fig. 3A), validating the IL-7 detection sensitivity of the methodology.
We demonstrated the high sensitivity of this methodology in the direct detection of cisplatin in urine samples and the fluorescence imaging of cisplatin in living cells.
Moreover, in order to investigate the sensitivity of the methodology to different crowding conditions, the proposed approach was applied in relation to three different levels of travel demand obtained by means of the first type of surveys, whose results, expressed in terms of a distribution function, are shown in Fig. 4.
This difference might be related to the higher sensitivity of the methodology applied nowadays.
To validate the specificity and sensitivity of this methodology, we applied the screening assay to cellular conditions and proteins of interest known to regulate macropinocytosis.
To investigate the sensitivity of LPv methodology, we examined 50 manually selected genes related to three functional categories: development, Alzheimer disease (AD) and cancer [14].
Whether this reflects a somewhat lower sensitivity of our methodology compared to Juliano et al remains to be determined, but it is certainly consistent with the conclusion that these recrudescences are not associated with selection of a minority, mutant Pfcrt clone.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com