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The interleaved transmissions ensure both a highly sensitive vector flow image and a high-resolution B-mode images.
Many of them are based on the concept of sensitivity, where the attacker tries to disclose the tangent hyperplane locally around a point (called sensitive vector) on the border of the detection region.
The PCR products and temperature sensitive vector pHY304 were ligated using T4 ligase to create the plasmids pHY5448RopB and pHY5628RopB.
The PCR fragments were then ligated into the temperature sensitive vector pHY304, [92]) and the resulting plasmids pKB8 (Δstp1::cm) and pKB1 (Δstkn::kn) were electroporated into S. aureus strain RN4220 using methods described previously [69].
Each flanking region for the LEE4 knockout was PCR amplified and cloned into the temperature sensitive vector pIB307 creating pHY3.
This technology requires flanking DNA to be cloned into a temperature sensitive vector but the resulting clone allows great flexibility for further modification of the target sequence.
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These small yet critical changes exemplify how sensitive vector-based dynamics are to slight temperature changes during conditions at Peak.
Following intracranial inoculation into nude mice, the miR-124-sensitive vector failed to replicate or show overt signs of pathogenesis.
This may be influenced by pre-adult mosquito life stage development impacting adult abundance and temperature-sensitive vector competence.
All selected colonies had lost the appropriate temperature-sensitive vector and the absence of New1 and/or ClpB was confirmed by Western blot analysis.
mutL or mutS sequences were obtained from BamHI and EcoRI-digested pGEM-T easy plasmids containing locked or unlocked alleles and subcloned into BamHI and EcoRI-digested pHSG415, a temperature-sensitive vector used for allele replacement via homologous recombination [ 26].
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