To further analyze early pathological forms of Tau in PNC, we performed a more sensitive AT8 staining, by omitting the stringent extraction of soluble forms of Tau, thereby staining both aggregated and soluble forms of AT8-stained Tau.
Compared with the control, more and longer fibrils of A β in the presence of geniposide were observed under electron microscope though the fibrils became less sensitive to thioflavin T staining.
You might want to use plastic bins for some items, like papers and clothes, which are sensitive to water and staining.
This "sensitive" method, however, fails to stain Pick bodies, another distinct tau-positive deposit [ 16, 85, 96, 117, 118].
Mander's coefficients are not sensitive to the intensity of staining as they are normalized against total pixel intensity; thus they can be employed when the staining of one antigen is stronger than the other.
Cells that became sensitive to trypan blue staining were monitored by using light microscopy or an automated cell counter (Countess, Invitrogen, Grand Island, NY, USA).
Aside from leather, natural wood is perhaps the most sensitive to damage and staining.
However, Congo red staining is neither very sensitive nor specific as it has been shown to stain amorphous aggregates [ 98] that can bind to cellular membranes [ 100].
In addition, more cells are stained with 2C8 than with mAb1 and mAb11, indicating that staining with 2C8 is more sensitive than staining with mAb1 and mAb11, used previously by our group [ 13- 15].
Flow cytometry experiments −using the propidium iodide to stain nuclear DNA or a more sensitive DNA stain− would be suitable to this direction.
The gels were stained with the highly sensitive silver staining approach according to Blum and colleagues [50].
