To explore why Gal4 was largely immune from silencing at HMR whereas Abf1 is sensitive, we tested whether mutant forms of Gal4 that compromise some aspect of its function would become sensitive to silencing.
Interestingly, HMVE cells were more sensitive to silencing of c-Src than HUVE cells since silencing of Src expression almost totally inhibited VEGF-stimulated proliferation (Figure 4G).
CAL51 and HeLa cell lines were significantly more sensitive to silencing of WEE1 than cell lines that do not overexpress WEE1 (Figure 4b and Figure S2).
While the effects of 16:0 and 20 4 LPC on COX-2 expression were profoundly sensitive to silencing of either c-Jun or p65 (NF-κB), respectively, silencing of cyclic AMP responsive element binding protein (CREB) attenuated markedly the effect of both LPC.
He next showed that primary cells from acute lymphoblastic leukemia samples that harbored t(1 19) were consistently sensitive to silencing of the cell-surface receptor ROR1, and to dasatinib, a kinase inhibitor, because of its inhibition of the pre-B-cell receptor signaling complex.
In another study, the sociologist Zhang Yi found that the new middle class was acutely sensitive to feeling silenced or to efforts to deprive it of information.
However, some drivers have found these systems too sensitive and turn them off to silence the beeps.
This screen uses a GFP transgene that is particularly sensitive to epigenetic silencing, to identify genes elsewhere in the genome that when mutated alter the extent of silencing at the transgene locus (Blewitt et al. 2005).
FA deficient cell lines were found to be sensitive to Chk1 silencing by siRNA compared to FA proficient cells [ 29].
We found that ECs were very sensitive to TIAM1 silencing, most likely due to the key pro-survival role of Rac1 in normal cells.
