Since retroviruses may be more sensitive to dNTP pool alterations than cellular polymerases, we hypothesized that gemcitabine would be an alternative to hydroxyurea that could be translated to clinical use for the treatment of retroviral infections when used in combination with current anti-retroviral therapies.
It is important to note that, while mtDNA is sensitive to nucleotide pool imbalance, nuclear DNA (nDNA) appears not to be.
Because 5-FU (which specifically depletes dTTP pools) causes a large increase in expression whereas TdR (which depletes all precursors except dTTP) does not, this mechanism might be particularly sensitive to dTTP pools.
We have previously studied myelin integrity using a novel MRI sequence sensitive to water pools within myelin and intra- and extra-cellular spaces, known as Multi-component Driven Equilibrium Single Pulse Observation of T1 and T2 (mcDESPOT).
A novel MRI sequence (mcDESPOT) sensitive to water pools within myelin and intra- and extra-cellular spaces was applied to 23 ALS patients, seven PLS patients and 12 healthy controls, with interval follow-up in 15 ALS and four PLS patients.
Measuring the impact of ER-ChaC1CtoS on glutathione levels required an assay that would be selectively sensitive to the ER pool of glutathione.
Sensitivity analysis by the California Air Resources Board confirmed that the mean and distribution of premature mortalities from long-term exposure to PM2.5 are not sensitive to the random-effects pooling of CR functions (Tran et al. 2009).
In addition, some phenotypes are sensitive to the genetic background and pooling of several mutants may not always be feasible.
More work is needed to identify which zinc pools are sensitive to changes in zinc intake and bodily stress, or to zinc loss, especially in children who are at risk of zinc-deficiency related disorders.
