Indeed, even SPR analysis is not sensitive enough to accurately measure the apparent Kd of the toxin-wild-type receptor complex and a more sensitive FRET-based assay involving the use of AF488 (donor fluorophore -labeled PA and Afluorophore -labeledoPAore)-landled AF5462 I domacceptorto be used in that study.
MMG provides a sensitive tool to accurately quantify mechanical responses to motor action potentials generated by electrical stimulus, allowing more reliable assessment of nerve decompression.
Due to the intrinsic weak or transient binding of fragments (mM μM in dissociation constant (KD)) to targets, methods must be sensitive enough to accurately detect and quantify an interaction.
However, sEMG signals lack sensitivity to detect changes in the number and discharge rate of active motor units that would indicate increased central motor output [108, 109] and therefore may not be sensitive enough to accurately determine whether or not altering pH may affect descending central drive.
However, to identify small changes in the density or duration of colonization, sensitive methods to accurately detect these changes will be important in estimating vaccine effects.
Specifically, it is not sensitive enough to accurately detect low, but statistically significant, fold changes of around 2-fold [ 23, 24] that are typical for miRNAs [ 25, 26].
However, the resolution of the images collected by a standard hospital CT scanner is not sensitive enough to accurately determine the thickness of the cortical bone.
The conventional means to assess the hemodynamic status in these infants such as heart rate, capillary refilling, pulse quality and non-invasive blood-pressure are not sensitive enough to accurately assess the magnitude of a hemodynamic imbalance.
Specifically, it is not sensitive enough to accurately detect low, but statistically significant, fold changes (<2.0) (Peirson and Butler, 2007; Vikalo et al., 2010) that are typical of the fine-tuning nature of miRNAs (Moazed, 2009).
