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We found that using the full microscope illumination path to generate structured illumination patterns is perfectly compatible with speckle illumination, while grid patterns – which can also be used in HiLo microscopy [25] – proved to be more sensitive to aberrations (in particular, distortion) introduced by the microscope lens L5.
As a result, laboratories attempting to expand hESC lines must subject them to frequent characterization tests sensitive to aberrations below the resolution of G-banded karyotypes.
The evidence strongly suggests that zones closest to the topological centre contain the largest numbers and proportions of important proteins, with zone 1 on the whole appearing to be most sensitive to aberrations.
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Since I had found that the turn is uniquely sensitive to aberrant midline crossing, I decided to use a genetic method to target these aberrations to specific segments of the nervous system.
An advanced telephoto lens design is employed in this research, which has the characteristic of being more sensitive to axial aberrations than lateral chromatic aberrations.
GFT, which is conceptually similar, at an abstract level, to the radial centroid transform (Yang and Parvin, 2003), is sensitive to morphometric aberrations and internal structure within the nucleus.
This is an expected result, as it is known from diffraction theory that the field confinement in the axial dimension is more sensitive to field aberrations than the field distribution in the lateral dimension [ 46, 47].
We also point out that wave-mixing processes such as FWM are sensitive to chromatic aberration induced by the microscope and the sample.
Because close to 65 000 individual loci are counted on chromosome 21 alone, it was open to debate whether this approach would be sufficiently sensitive to detect more subtle chromosomal aberrations such as the Robertsonian translocation between chromosomes 21 and 15 (or 14), which has a role in 2 3% of cases with DS (Ref. 1).
There are two more factors: one is that this kind of laser lithography system is not sensitive to the influence of the spherical aberration; another is that the objective is designed as an aplanatic lens which eliminates the spherical aberration of the objective.
TDI has been shown to be sensitive to identify anthracycline-induced cardiomyopathy and aberration in TDI parameters may be identified before abnormalities can be detected by conventional echocardiography [ 46].
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Justyna Jupowicz-Kozak
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