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Then the replication products of long duplex DNA probes were stained with a sensitive reagent SYBR Green I (SG).
Hence, it is suitable as a sensitive reagent in biochemical detection experiments, especially when produced as a functional fusion protein with alkaline phosphatase as reporter enzyme.
The purpose of this study was to design a novel in vitro tool by using recombinant protein technology to detect specific antibody responses against Toxoplasma gondii in a simple, rapid and highly sensitive reagent.
The 'PROMPT' operation causes a pop-up message to be generated during a production run with instructions for the user when non-automated tasks are required, such as the preparation of a sensitive reagent for addition through an external addition line.
The use of ESAT-6 and CFP10 as skin test reagents have already been described in this model, in which a combination of these two molecules proved a specific and sensitive reagent for the detection of TB infection [10].
Plasma samples were tested as recommended by the scientific and standardization subcommittee for LA of the ISTH.13 Screening for LA included activated partial thromboplastin time (aPTT, performed with LA sensitive reagent), dilute prothrombin time (1/500 dilution of thromboplastin in CaCl2), and dilute Russell's viper venom time (dRVVT).
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Highly sensitive signal-off ECL detection was shown by combining electrode generated substrate ECL technique with the use of magnetic nanoparticles coated with catalase for a construction of sensitive reagent-less sensing system.
The system also has an integrated, automated reagent handling robot to deliver sensitive reagents from sealed vials on demand.
In addition, keeping the atmosphere away from moisture that may affect the moisture sensitive reagents decreases the possibility of formation of the undesired byproducts.
The ELIXYS automated radiosynthesizer is capable of performing radiosyntheses requiring demanding conditions: up to three reaction vessels, high temperatures, high pressures, and sensitive reagents.
All operations involving air sensitive reagents and materials during catalysts precursor synthesis were carried out under nitrogen atmosphere using standard Schlenk, vacuum or dry box techniques.
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