Exact(20)
Cold sensitivity can be re-introduced into squirrel and hamster TRPM8 by transferring the transmembrane domain from the cold sensitive rat ortholog.
Sensitive Rat Insulin radioimmunoassay kit was purchased from Linco Research, Inc. (St. Charles, MO).
Insulin content and secretion were assessed with the Sensitive Rat Insulin RIA Kit (Linco Research) as described [68].
Insulin levels were measured in both the media and the pellet by an ultra sensitive rat insulin ELISA (Crystal Chem Inc., Downers Grove, IL, USA).
We measured glucose levels with a Glutest Pro (Sanwa Kagaku Kenkyusho Co., Japan), insulin and leptin by sensitive rat insulin RIA kit (SRI-13K, Millipore, Billerica, MA) and mouse leptin RIA kit (ML-82K, Millipore, Billerica, MA).
Serum glucose concentrations were measured by a commercially available kit using the glucose oxidase method (Pointe Scientific Inc., Canton, MI, USA), and insulin levels were measured by an ultra sensitive rat insulin ELISA (Crystal Chem Inc., Downers Grove, IL, USA).
Similar(40)
Histopathology verified that these values truly reflected hepatotoxicity of sensitive rats within their treatment groups (Figure 1A and 1B).
Of particular interest was the decreased expression of Hsd11b1 and Slc27a5 only in sensitive rats.
With regard to rat models that mimic menopause, both SHR and Dahl salt sensitive rats have been used.
Of these, only three were altered in resistant rats but not in sensitive rats (Il1r1, Phyh, Hacl1).
For example, Dahl salt sensitive rats exhibit hypertension with aging or more rapidly with a high salt diet [ 90].
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