Sentence examples for sensitive origin from inspiring English sources
The rescue plasmid was created by PCR cloning MSMEG_6382 into the vector pCG76, which carries a temperature sensitive origin of replication for M. smegmatis and a streptomycin resistance gene [16].
The vector pG+host8 containing a temperature sensitive origin of replication was used to introduce degS S76A and S76D point mutations in situ, replacing the chromosomal version of degS in B. subtilis 168 [43].
It also has a temperature sensitive origin that is functional at 30°C.
Here we report on the generation of allelic exchange mutants using a double negative-selection system utilizing a temperature sensitive origin of replication of plasmid pAL5000 and the Bacillus subtilis sacB gene.
E. coli DB3.1 was co-transformed with pWY189 and pKD46 which encodes the bacteriophage lambda Red homologous recombination functions regulated by arabinose induction and possesses a temperature sensitive origin of replication [ 41].
This plasmid comprises the TMDH transposon cassette, a temperature sensitive origin of replication for growth in S. aureus (stable replication at temperatures of 30°C or below), and a chloramphenicol resistance gene for selection in S. aureus at 5 μg/ml.
Due to its thermo-sensitive origin of replication, the plasmid can be eliminated at non-permissive temperatures, and markerless deletion mutants can be obtained.
This fragment was then subcloned into pPR27, a vector with a temperature-sensitive origin of replication for M. smegmatis, a gentamycin resistance gene and a gene encoding sucrose sensitivity, sacB (Fig. 3A).
The plasmid has a temperature-sensitive origin of replication and can replicate at 30°C but not at 42°C and is cured from the bacterial cells over several generations of growth at 42°C [16].
Incubation at 39°C precludes the replication of the temperature-sensitive origin of replication of pVAP39.
pKD46 encodes the λ Red recombinase genes under control of the araB promoter and a temperature-sensitive origin of replication.
