Sentence examples for sensitive mutation from inspiring English sources

High-resolution melting curve analysis (hrMCA) has recently been developed as a post-PCR mutation scanning method which enables simple, rapid, cost-effective, and highly sensitive mutation screening of large genes.

Despite the development of sensitive mutation detection approaches, a thorough validation of these in a clinical setting has so far been lacking.

These cells have a temperature sensitive mutation in TAF1 which functions normally at the permissive temperature, 32°C, but is inactivated at the non-permissive temperature, 39°C [45].

We performed, in a clinical setting, a systematic validation of dideoxy 'Sanger' sequencing and pyrosequencing against massively parallel sequencing as one of the most sensitive mutation detection technologies available.

Several methods were established to offer sensitive mutation detection, all including DNA extraction, PCR amplification and subsequent mutation analysis by sequencing or genotyping-based assays [10], [11], [12], [13], [14], [15], [16], [17], [18], [19].

These larvae have a temperature sensitive mutation in the HSF DNA binding domain which prevent HSF from binding to HSEs and inducing HSP gene transcription at non-permissive (i.e. heat shock) temperatures [14].

Patient tissue was analyzed for the presence of BRAFV600E mutation with both conventional DNA sequencing and the more sensitive mutation-specific PCR (MSPCR) as described previously [6], [22].

The conditional-lethal vaccinia virus mutant ts4149, which harbors a temperature-sensitive mutation in the D4R (uracil-DNA-glycosylase) open reading frame, was kindly provided by G. McFadden (University of Western Ontario) [51].

G418R cell lines were screened for their ability to complement the growth of ts4149, a vaccinia virus mutant that harbors a temperature-sensitive mutation in the udg (D4R) gene, at the non-permissive temperature of 39.5°C [51], [53].

This repressor, referred to as Maf1, was originally identified in S. cerevisiae by the isolation of a temperature-sensitive mutation, maf1-1, thaffectedtRNAtRNA suppressor efficiency and interacted genetically with pol III [16].

The ky51 allele is a temperature-sensitive mutation in the GTPase domain of DYN-1, resulting in a rapid and reversible functional inactivation of the protein at the restrictive temperature [20].