Sentence examples for sensitive mismatch from inspiring English sources

We used chemically sensitive mismatch repair null cells to allow for the rapid selection of resistant variants combined with whole genome sequencing methods to identify the genetic alterations responsible for drug resistance.

The generation of S1 nuclease sensitive mismatch 'bubbles' is likely to have a broader application than mtDNA haplotype discrimination alone, including alternative SNP detection strategies and other manipulations that require sequence specific cleavage of DNA, such as the isolation of specific genomic sequences for cloning in the absence of unique restriction sites.

Moreover, typical thin film PV modules are less sensitive to mismatch than crystalline silicon based ones.

It is shown that, in this state, the phase difference between the two resonators is highly sensitive to mismatch and can be used to sense changes of physical quantities, whereas it is largely unaffected by drift (e.g. induced by unwanted changes in environmental conditions).

Probes with high GC content, however, were not sensitive to mismatches and outperformed probes with optimal GC content when there were several mismatches between the hybridizing DNA fragment and the probe [ 41, 48].

However, their performance is sensitive to mismatches in the analog components.

Shortening the length of the guide RNA makes it more sensitive to mismatched sequences, but it can also create entirely new off-target effects.

On the other hand, Figure 3 shows that, when, a smaller value of renders the performance less sensitive to mismatched signals.

For example, sgRNA with a truncated base-pairing sequence (17 nt instead of 20 nt) enhanced the targeting specificity because truncated sgRNAs have reduced binding affinity with the target DNA and thus are more sensitive to mismatches (Fu et al., 2014).

Extension of the probe increases the specificity of this process even further since polymerases are sensitive to mismatched bases at the 3' of oligonucleotides.

T4 DNA ligase is especially sensitive to mismatches on the upstream side of the ligation junction (Fig. 6C), and this sensitivity provides the basis for diagnostic tests for single nucleotide polymorphisms [31], [36], [37].