Rapid activation by ROC of JNK (as measured by SP600125-sensitive JUN phosphorylation) and MEK/ERK signaling (as measured by UO126-sensitive increase in phosphoERK) was observed and the 4 most potent rocaglates had similar effects (Fig. 6).
Correspondingly, the liver responds to mTORC1 activation with a rapamycin-sensitive increase in PGC1α, a key regulator of mitochondrial biogenesis, which is normally induced under fasting conditions to facilitate glucose production.
Here we show that consolidation of inhibitory avoidance (IA) LTM entails mTOR activation in the dorsal hippocampus at the moment of and 3 h after training and is associated with a rapid and rapamycin-sensitive increase in AMPA receptor GluR1 subunit expression, which was also blocked by intra-hippocampal delivery of GluR1 antisense oligonucleotides (ASO).
Taken together, these data indicate that, in bovine chromaffin cells, the P-CTX-1B-induced activation of voltage-sensitive Na+ channels produces a TTX-sensitive increase in intracellular Ca2+ due to both influx of Ca2+ into cells and release of Ca2+ from internal stores.
Previous findings showed that there is IOP-sensitive increase in Aβ in glaucoma [ 14– 17].
Placing naive cells in a hypoosmotic environment results in a time-dependent and HgCl2-sensitive increase in cell volume.
Exposure of the cultured cells to D-Asp or THA results in a rapamycin-sensitive increase in 4E-BP phosphorylation.
Indeed, incubation of untreated HUVECs with A23187 triggered an l-NNA-sensitive increase in dihydroethidium fluorescence that was abolished upon loading the cells with BH4 (Fig. 5).
Of further note was our observation of a MOG-sensitive increase in expression of B2m at day 15 p.i. in CP stromal capillary tissue, and at both time-points in the choroidal epithelial tissue.
When CHIP28 was expressed in Xenopus oocytes, a mercurial-sensitive increase in the water permeability of the plasma membrane was observed, demonstrating that CHIP28 was a long-sought water channel protein (Preston et al. 1992).
