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To titrate the expression of the fusion protein, GFP-BAX was cloned into a tetracycline sensitive expression cassette and cotransfected with a plasmid expressing the rtTA transcription factor into HCT116 BAX-/ cells.
To identify miRNAs that are differentially expressed in tumors and tumor subtypes, we carried out highly sensitive expression profiling of 735 miRNAs on samples obtained from a statistically powerful set of tumors (n = 80) and normal colon tissue (n = 28) and validated a subset of this data by qRT-PCR.
Our results suggest that the RNA editing process responds to temperature alterations via two distinct molecular mechanisms: through intrinsic thermo-sensitivity of the RNA structures that direct editing, and due to temperature sensitive expression or stability of the RNA editing enzyme.
He has an alert, sensitive expression, accented by a widow's peak.
A song that is really about a deep-seated fear of commitment remained a sensitive expression of timidity and ambivalence, lacking in terror.
We tested this and show that with the act-GAL 4 driver, the temperature sensitive expression of GAL4 is quite pronounced.
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Yet it doesn't take much imagination to think what else certain companies and developers might really want to use real-time tracking of facial expressions for: Hyper sensitive expression-targeted advertising and thus even more granular user profiling for ads/marketing purposes.
To see why this might be necessary for an adequate account of meaning, let's focus on context sensitive expressions like 'here'here' or 'this'this
Contextualism about knowledge, after all, can hardly get off the ground unless 'knows' really is a context-sensitive expression.
But in sentences in which there is no pronounced second argument, as in 'George is rich', the predicate 'rich' is accompanied by an unpronounced occurrence of a context-sensitive expression that resembles a pronoun.
Minimalists try to keep context-sensitive expressions to a minimum — Cappelen and Lepore (2005, 2007) are in this camp — and those who pose a context-sensitive expression whenever is needed.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com