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The results show the parameters can be divided in to strongly sensitive, conditional sensitive and weak sensitive parameters according to its effect on the cell polarization curve.
The construction of the JH2-2 derivative mutants was carried out with the pMAD plasmid by exploiting its property of thermo sensitive conditional replication [54].
We sought to extend this technology specifically for Asian populations, and therefore designed and synthesized 30 UV-light sensitive conditional ligands, and constructed the corresponding HLA molecules.
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The necessary effect of information granulation is accomplished in the framework of fuzzy sets, especially via context-sensitive (conditional) fuzzy clustering.
Thus, YBP2 has a temperature-sensitive conditional synthetic-lethal interaction with spindle checkpoint genes.
The hopTum-l mutants provide a convenient system to test this, as the mutation is temperature-sensitive conditional.
Consistent with these observations, ybp2Δ exhibited temperature-sensitive conditional synthetic-lethal interactions with ame1-4 or okp1-5, respectively (Figure 5G, bottom).
Figure S1. A. Design of UV-sensitive conditional ligands.
Synthetic genetic interactions were used to construct an essential synthetic genetic network using temperature-sensitive conditional alleles.
We found that the mcm5 and mcm4 PS1-hp mutants behave differently in that the mcm5 mutant was more deleterious with a higher plasmid loss defect and displays both temperature-sensitive and cold-sensitive conditional phenotypes.
Furthermore, mutants of Escherichia coli K12 which were defective in phosphatidylserine synthetase, were isolated as temperature-sensitive, conditional lethals [ 42]. * kdtA: 3-deoxy-D-manno-octulosonic-acid transferase is the enzyme of kdtA.
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