Wg overexpression with sd-Gal4 increased expression of the Wg target gene senseless (Figure 4O).
Conversely, in a gain-of-function situation MGM overexpression was able to activate the Wg target senseless (Figure 2J).
MGM increased the number of cells expressing Senseless (Figure 2A 2C insets; Figure S4), as well as the size of the wing pouch.
Boundary cells lacking Notch activity express Senseless (Figures 3E' and 3F').
Finally, when our in silico wing primordium lacks Cut activity (either in clones of mutant cells or in a mutant background), boundary cells start responding to the Wg signal, as shown by the expression of Senseless (Figures 4andand 4B') and ectopic activation of Notch in non-boundary cells is now unable to block the Wg signaling pathway (Figure 4C').
"People want you to make sense out of the senseless and help them figure out how God could still be a part of that, or at least present in it," he said.
Conversely, knockdown of Mad with RNAi (see below) caused decreased Senseless expression within clones (Figure 2H and 2I), which were accompanied in the adult wing by losses of the anterior wing margin within clones (Figure 2U).
As the name suggests, materialists historically held that everything was matter — where matter was conceived as "an inert, senseless substance, in which extension, figure, and motion do actually subsist" (Berkeley, Principles of Human Knowledge, par. 9).
Note that the in silico figures (and movies) show Senseless protein expression, although this species was not considered in our modeling approach.
Indeed, expression of wingless with patched-GAL4 in a domain along the A/P boundary, perpendicular to its normal expression domain, caused ectopic expression of canonical target genes, such as senseless (sens) and distalless (Dll) (arrowheads in Figure 2E and 2F).
In the first type of experiments, expression of Wg and Senseless was monitored after ectopic activation of the Notch signaling pathway (Figure 3C') or ectopic expression of Wg (Figure 3D') in a stripe perpendicular to the DV boundary.
