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For semen sampling, estrus was induced in a crossbred ewe with 2 mg of estradiol cypionate (ECP – Pfizer, São Paulo, Brazil) intramuscularly 48 h before semen sampling.
Both groups underwent a 2-month period of adaptation and training for semen sampling with artificial vagina.
They found a significant negative correlation between sperm concentration and O3 during the 0– to 9–, 10– to 14–, and 70– to 90 day periods before semen sampling.
The estimated air pollution and temperature time series within each county was then linked to the 90 day period before semen sampling for each subject.
Based on the concentrations of four regulated THMs (THM4), nine HAAs (HAA9), and total organic halides (TOX) measured weekly in tap water sampled from the distribution systems (Savitz et al. 2006), we calculated mean levels of DBPs over the 90 days preceding semen sampling.
Of the multiple analyses performed, the only statistically significant adverse association observed was between increased PM2.5 averaged over the 0– to 90 day period before semen sampling and an increase in the percentage of sperm with abnormally shaped heads and the percentage of sperm with cytoplasmic droplets.
Similar(51)
Semen parameters were measured for fresh semen samples (day 0) and for semen samples after 3 days of liquid storage (day 3) (Table 1).
There was nothing, except semen samples.
The semen sample was badly degraded, but still usable.
But there was no match with the semen samples.
The instructions note: "Seal before spin" – preferable to spraying a semen sample over nearby surfaces.
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