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These conditions were: diluent (IGGKPh, SPh or Tselutin); dilution rate (1 3 vs. 1 4); cooling time (45 vs. 60 min); dimethylacetamide (DMA) concentration as cryoprotectant (6 vs. 8%); equilibration time in DMA (1 vs. 5 min); semen drop volume (50 vs. 80 μL) and thawing temperature (60 vs. 75 °C).
Cell counting was performed on a slide mixing a drop of semen and eosin solution (semen drop plus eosin drop were placed between slide and cover slide) under 400 X magnification in a bright field microscope.
The method of feathering (the semen drop was spread along the back edge of the angled slide and pulled forwards over the slide to form the smear) was used for preparation of semen smears for assessing sperm morphology.
Similar(55)
He sent his semen to his queen with an eagle but, due to a fight with another eagle, the semen dropped into the river and was swallowed by the cursed Adrika-fish.
But biotechnology group Genus - best known for its bull semen - has dropped 10% to £17.37 after an unfavourable verdict in a US patent litigation case.
Gary takes cow semen orders and drops them off, occasionally doing the breeding himself ("anywhere from a wrist to your shoulder" is how far you have to stick your hand inside a cow, he says).
When the concentrated semen solution was dropped onto the surface of dry ice or metal-plate, the concentrated cryoprotectant crystallized and formed a spherical pellet regardless of the solution volume used.
During the initial data management process, case submissions and test requests associated with semen specimens were dropped, as they were felt to represent herd monitoring for PRRSV instead of a disease investigation process.
Even if methodological changes account for some of the observed drop in semen quality, they may not account for all of it.
A gorgeous jock putting on a wifebeater caught my attention as did the drop of semen resting above his lip.
The sages' wives in turn drop the semen into the Ganges river (the Ganga), where it flows to the shore from which Kārttikeya springs.
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