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Owing to the relatively low level of sequence similarity between probes and transcripts in cross-species hybridization, more probes might be considered to cross-hybridize in cross-species hybridization as compared to self species hybridization (Bar-or et al. 2006).
A random clade was manually chosen from the tree, as long as it met the following criteria: (1) 3–8 self species that formed a clear monophyletic group; (2) 10–20 close species that were closely related to the self clade; (3) the self and close species together formed a clear monophyletic group that was independent from all other species (the distal group).
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Recent population genomic studies also raise doubts about the long-term adaptive potential of self-fertilization, providing evidence that purifying selection is less effective in highly selfing species.
Population genetic theory predicts, for example, lower diversity in selfing species both within populations [34], [86], and often species-wide [87], [88].
This diploid selfing species is widely distributed in central Eurasia, the center of its distribution being in the southern coastal region of the Caspian Sea and Azerbaijan [6].
The ρ/θ ratio predicted in a selfing species is 1/ 1-F) times smaller than in an equivalent outcrosser, where F is the inbreeding coefficient [89].
If cross-population sweeps are common in A. thaliana, selection – especially in conjunction with increased LD in a selfing species – may contribute substantially to the observed excess of rare mutations seen in species-wide samples of A thaliana.
Because admixture may have significant influence on genetic structure even in selfing species [34] [35], the cpDNA genealogy and inferences about the evolution of the short-flowering-time phenotypes require further testing with nuclear gene sequences.
Selfing Species: Strictly selfing species do not outcross, and so outbreeding depression will not occur.
However, most selfing species have at least occasional outcrossing.
As expected for a selfing species, genotypes were highly homozygous.
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