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Single self experiments have been categorised as self indulgence or abuse, and trivial interventions masquerading as research studies as a source of amusement.
Single self experiments are common in all fields of medicine except clinical epidemiology There are more things in heaven and earth than are dreamt of in your clinical epidemiology handbook An example of self experimentation in clinical epidemiology A novel study design Not much else Cite this as: BMJ 2011 343 d7653.
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The test used virtual self-self experiments to derive intensity-dependent cutoffs.
The average SD of signal ratios from self-self experiments was 0.08.
However, if self-self experiments were repeated using the same sample, a number of genes appeared as significantly differentially expressed.
To monitor the consistency of the array experiments, "self-self" experiments were performed using the same sample as reference.
To estimate reliability, the HTself method used virtual self-self experiments (i.e., G3 cancer vs. G3 cancer) to derive intensity-dependent cutoffs.
To ensure microarray data baseline robustness, differentially labelled DNA from the S288C strain were co-hybridized, in a total of six self-self experiments, and used as controls.
Self-self experiments were used for data in Figure 1, Figure 2 (right), Figure 5, Figure 6, Figure 7B, Table 2, and Table 3.
If the signal ratios from two of these self-self experiments were inverted, no differentially expressed genes were found by SAM.
Significantly differentially expressed genes were determined by estimating the false discovery rate using a null distribution obtained from the self-self experiments, as described in the Background section.
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