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For 60 minutes under the Centre Court roof, Sharapova was her old young self, blasting forehand winners down the lines and serving with the fearlessness of the teenager who stared down Serena Williams to win the 2004 Wimbledon title.
It's not hard to imagine a bookish version of her younger self blasting New Order and disappearing between the pages of a medical history tome.
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Self BLAST of this dataset produced only 625 matches with a value of e−100, indicating a low level (<3%) of redundancy in the assembly of the reads.
A self BLAST of the sequences was performed to remove redundancy.
A self BLAST of these sequences followed by manual editing to remove the redundancy finally resulted in identification of 75 MAPKKK genes.
This gene set was filtered using custom python scripts to identify and remove alternative splice variants and recent gene duplications by self BLAST.
Self BLAST of this dataset produced only 281 matches with a value of e-100, indicating a low level (<1.5%) of redundancy in the assembly of the reads.
A self BLAST of these sequences followed by manual editing to remove the redundancy has finally identified 75 MAPKKK genes from the B. distachyon genome (Additional file 1).
In addition self BLAST of the sequences was carried out to remove the redundancy and then they were analysed for the presence of domains.
A self BLAST of these sequences followed by manual editing to remove the redundancy finally resulted in the identification of 75 MAPKKK genes.
These were self BLAST filtered as above, but at the protein level (BLASTp), with removal of the shorter sequence when amino acid identity > 90% over 33 amino acids with an eval < = 1x10-6.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com