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The selector pool of mRNA, extracted in this work from Ancistrus dolichopterus, is biotinylated to allow subsequent separation by magnetic particles coated with streptavidin.
This is achieved by rescuing the fraction of ss cDNA that disanneal only at very stringent conditions, which guarantees an almost perfect complementation between the probed and selector pool of transcripts.
Moreover, the species from which the selector pool of mRNA is extracted may be selected in order to increase the level of conservation of the enriched cDNA library: the more evolutionary distant the selector species will be, the more conserved the isolated transcripts will be.
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Selectors share a pool representing the weak classifiers, which are denoted by h w (w = 1,…,W).
The pool of biotinylated selector mRNA (7 μl) and the pool of first strand cDNA of the species of interest (7 μl) are mixed and the total volume is adjusted to 15 μl.
The committee might have wanted to enlarge the pool of potential selectors by making the jobs full-time.
Some of the region's increasing pool of talented selectors will also be contributing to the evening, including master diggers Last Waltz, as well as Jammhot and Cubik.
If the action executed by the SR-Browser did not generate the expected traffic (for example when the action were not triggered during the session), the next action selector chooses another action from the pool of candidates, and assigns it to the SR-Browser (lines 18-19).
It gave the selectors confidence that they had the right pool of players to persevere with.
In the ISSH approach, the transcriptome of the species of interest ("probed" species) is reverse transcribed into single stranded (ss) cDNA and then hybridized against the biotinylated mRNA pool of a distinct yet close relative ("selector" species).
Phage-Abs from each selected pool were individually screened by cell-ELISA for their ability to bind the selector cell line.
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