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The influence of two factors, chiral selector concentration and organic modifier content, on the responses was modelled.
Critical parameters such as chiral selector concentration, pH and temperature have been studied to evaluate how they affected responses such as resolution and migration times.
Robustness was tested using a full factorial design for the following four experimental variables varied at two levels: pH of the BGE, chiral selector concentration in the BGE, phosphoric acid concentration in the BGE, and temperature.
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Different selector concentrations were tested at various pH levels (pH 2.5, 4.0, 6.0, 9.0) to optimize the separation conditions.
HS-β-CD and HS-γ-CD were found to be the most effective chiral selectors with a shorter retention time at low selector concentrations.
The experiments were performed according to a face centered cube response surface experimental design for obtaining information how the factors such as concentration of the chiral selector, pH, buffer concentration and voltage affected the two response goals, resolution and analysis time.
The concentration of the chiral selector and organic modifier, pH of background electrolyte (BGE), capillary temperature, and applied voltage were systematically optimized by using orthogonal design and concentration of chiral selector was further optimized.
The basic chemical and instrumental parameters of enantioseparation as concentration of buffer, chiral selector and organic additive, pH, temperature and applied voltage were optimized afterwards using an orthogonal experimental design.
Four factors affected the separation: buffer concentration, pH of the buffer, concentration of the chiral selector and the applied voltage.
Initially, different chiral selectors were systematically investigated and then a experimental design with three quantitative factors (cyclodextrin concentration and background buffer pH and concentration) were evaluated.
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