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Well isolated single colonies were selectively marked and propagated twice to check the purity and stored at −80°C with 20% sterile glycerol for further investigation and propagated twice in the same medium at 37°C before use.
The latter recognizes 5-methylcytosines, which are very abundant in mouse constitutive heterochromatin around centromeres, and thus, pericentric heterochromatin was very selectively marked by green fluorescence (Fig. 1 B, central panel).
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The Apt-PTX-PLGA NPs treated cells were stained with tubulin marker to selectively mark the micro-spindles.
The results demonstrate that the probe can selectively mark lysosomes and is valuable to monitor intracellular pH in living cells.
By using a genetically engineered mouse in which the lacZ coding region substitutes and reports for the ephrin-B2 coding region, we demonstrate that ephrin-B2 expression continues to selectively mark arteries during later embryonic development as well as in the adult.
Finally, Robo4 is a homolog of the brain-specific Roundabout receptors that bind the secreted Slit ligands and play roles in axon guidance, while PRCR selectively marks HSC [37] and Prion protein has been implicated in HSC self-renewal [38].
The latter probe (Ahd2) selectively marks the most lateral/rostral mdDA subset (Jacobs et al., 2007).
Recently, the novel D2-40 antibody, which selectively marks endothelium of lymphatic vessels, was released.
To discriminate human nuclei that were incorporating BrdU (BrdU+ hB, green), we used gamma satellite probe (γ-satellite, red) to selectively mark mouse nuclei.
These data indicate that Dlk1 selectively marks a peripheral fetal hepatocyte-like subpopulation, whereas HAI-1 is expressed in cholangiocytes in normal liver as well as in the ductular epithelial cells of the entire HPC response.
We next monitored the internalization of surface-biotinylated EGFR in response to EGF followed by quantitative analysis by densitometry, as biotinylation is widely used to selectively mark surface-exposed proteins including EGFR without reported alterations of their trafficking or function (Choi et al, 2004; Elia, 2008).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com