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This microscope is a multi-view selective illumination (MuVi-SPIM) microscope.
In summary, a heated region is generated by the selective illumination of the absorbing substrate with a light source.
Photoswitching fluorescent proteins, which change color upon selective illumination, including Kaede (Ando et al. 2002; Owens and Edelman 2015), Dendra2 (Pham et al. 2012) and Eos (Wiedenmann et al. 2004) have also been used to visualize mitochondrial dynamics.
He provided a "correction to the Original Bibliographic Citations," complete with diagrams, for a theatrical stage technique that used selective illumination and a sheet of plate glass inclined at 45° to give an illusion of the nymph Amphitrite rising from the sea and floating in air, or of an audience volunteer dissolving to a skeleton and back again.
This in turn makes measurements of superficial structures and the selective illumination of deeper regions significantly more complicated.
We use TIRF microscopy here not to provide a selective illumination of events very close to the plasma membrane but instead with an increased penetration depth to facilitate this increase in signal-to-noise.
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Fluorescence light sheet microscopy, such as selective plane illumination microscopy (SPIM; Huisken et al., 2004), has proven to be a powerful tool to image developmental processes in vivo with fast, high-resolution optical sectioning over large volumes (Huisken and Stainier, 2009).
In order to enable super-resolution imaging of whole living organisms, novel long-term continuous imaging methods with high spatial resolution and minimal photodamage such as selective plane illumination microscopy (SPIM) (Huisken et al. 2004) or Bessel beam plane illumination (Gao et al. 2012; Planchon et al. 2011) are urgently needed.
The technique, called microenvironmental selective plane illumination microscopy (meSPIM), uses exceptionally long, thin beams of laser light to trigger fluorescence in a sample, causing it to glow.
Panier, T. et al. Fast functional imaging of multiple brain regions in intact zebrafish larvae using Selective Plane Illumination Microscopy.
Panier, T. et al. Fast functional imaging of multiple brain regions in intact zebrafish larvae using selective plane illumination microscopy.
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